Mixed-agonist action of excitatory amino acids on mouse spinal cord neurones under voltage clamp.
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چکیده
منابع مشابه
Agonist- and voltage-gated calcium entry in cultured mouse spinal cord neurons under voltage clamp measured using arsenazo III.
Spinal cord neurons is dissociated cell culture were loaded with the calcium indicator arsenazo III using the whole-cell patch-clamp recording technique. Under voltage-clamp, depolarizing voltage steps evoked transient increases in absorbance at 660 nm, with no change at 570 nm, the isosbestic wavelength for calcium-arsenazo III complexes. The optical response occurred with a threshold depolari...
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Fluorescence histochemical studies have demonstrated a topographical distribution of dopaminergic nerve terminals in limbic forebrain and cortical regions4, 9-11. These data, together with biochemical evidence indicating significant amounts of dopamine (DA) in these regionsl,XS, zl, suggest the existence of a major anatomical pathway projecting to forebrain areas which originate in mesencephali...
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Acute administration of a single dose of NMDA on embryonic day (E) 7 or later induces a marked excitotoxic injury in the chick spinal cord, including massive necrotic motoneuron (MN) death. When the same treatment was performed before E7, little, if any, excitotoxic response was observed. Chronic treatment with NMDA starting on E5 prevents the excitotoxic response produced by a later "acute" ad...
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[Ca2+]i was measured using fura-2-loaded isolated catfish horizontal cells in the presence of L-glutamate and the glutamate analogs kainate (KA), quisqualate (QA), and NMDA. Caffeine was used to release Ca2+ from intracellular stores. Cell membrane potential was controlled with a voltage clamp to prevent activation of voltage-dependent Ca2+ channels in the presence of agonist. All excitatory am...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1984
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1984.sp015360